Feline Calicivirus Antigen Rapid Test (FCV Ag)

Feline Calicivirus (FCV) Antigen Rapid Test Kit is a chromatographic immunoassay used for the qualitative detection of Feline
Calicivirus antigen in feline secretion of eye mucus (in the conjunctiva), nasal discharge and saliva.
After the aluminum foil bag is opende ,it is recommended to use it within 1 hour.

PRINCIPLES
Feline Calicivirus (FCV) Antigen Rapid Test Kit is a chromatographic immunoassay used for the qualitative detection of Feline
Calicivirus antigen in feline secretion of eye mucus (in the conjunctiva), nasal discharge and saliva.
The invisible anti-FCV antibody (T line) and secondary antibody (C line) are immobilized on a nitrocellulose membrane in result window.
Another anti-FCV antibody is conjugated with colloidal gold particle and immobilized on glass fiber as conjugate pad.When specimen is
applied into the sample well on device, FCV antigen will bind to the gold conjugate and migrate on the surface of test strip by passive
diffusion,and a visible T line will appear in result window after 10 minutes. The C line has to appear every time when a test is properly
performed.

MATERAILS PRAVIDED
1. Test Devices: 10 pieces test devices individually pouched.
2. Assay diluent in tube:10 units
3. Disposable swab:10 pcs
4. Disposable dropper cap:10 units
5. Work station:1 unit
6. Instructions For Use: 1 copy attached.

TEST PROCEDURE
1. The test device, specimen, assay diluent should be brought to room temperature (15~30°C) prior to use.
2. Place the assay diluent tube on the work station and tear off the aluminum film.
3. Collect feline eye mucus(in the conjunctiva), nasal discharge or saliva with a swab, then insert the swab into the assay diluent tube
immediately.
4. Mix the swab until the specimen dissolves completely into the assay diluent (Approximately 10sec), then squeeze the solution from
the cotton swab into the assay diluent tube as much as possible.
5. Discard the cotton swab and cover the tube with dropper cap. Wait for 2 minutes.
6. Remove the test device from the foil pouch and place it on a flat and dry surface.
7. Add 3 drops of assay diluent drop by drop into the sample well on device vertically and start the timer.
8. Read the results at 10~15 minutes. Any results after 15 minutes are no longer valid.

INTERPRETATION OF THE RESULT
Positive result:
Test line (T) and control line (C) within the result window indicate the presence
of FCV antigen.
Negative result:
Only control (C) line appears in the result window indicating that FCV antigen
has not been detected.
Invalid result:
If the control (C) line does not appear, the result might be considered invalid.
The specimen should be retested.