PRINCIPLES
Giardia (GIA) Antigen Rapid Test Kit is a chromatographic immunoassay for the qualitative detection of Giardia antigen in
canine or feline stool(feces).
The invisible anti-GIA antibody (T line) and secondary antibody (C line) are immobilized on a nitrocellulose membrane in result
window.Another anti-GIA antibody is conjugated with colloidal gold particle and immobilized on glass fiber as conjugate pad.When
specimen is applied into the sample well on device, GIA antigen will bind to the gold conjugate and migrate on the surface of test strip
by passive diffusion, and a visible T line will appear in result window after 10 minutes. The C line has to appear every time when a test is
properly performed.
MATERAILS PRAVIDED
1. Test Devices: 10 pieces test devices individually pouched.
2. Assay diluent in tube:10 units
3. Disposable swab:10 pcs
4. Disposable dropper cap:10 units
5. Work station:1 unit
6. Instructions For Use: 1 copy attached.
TEST PROCEDURE
1. The test device, specimen, assay diluent should be brought to room temperature (15~30°C) prior to use.
2. Place the assay diluent tube on the work station and tear off aluminum film.
3. Collect canine or feline feces using a swab, then insert the swab into the assay diluent tube immediately.
4. Mix the swab until the specimen dissolves completely into the assay diluent (Approximately 10sec).
5. Squeeze the solution from the cotton swab as much as possible, then discard the cotton swab and cover the tube with dropper cap.
6. Wait for 2 minutes to precipitate the large particles.
7. Remove the test device from the foil pouch and place it on a flat and dry surface.
8. Add 3 drops of mixed specimen drop by drop into the sample well on device vertically and start the timer.
9. Read the results at 10~15 minutes. Any results after 15 minutes are no longer valid.
INTERPRETATION OF THE RESULT
Positive result:
Test line (T) and control line (C) within the result window indicate the presence
of GIA antigen.
Negative result:
Only control line (C) appears in the result window indicating that GIA antigen
has not been detected.
Invalid result:
If the control line (C) does not appear, the result might be considered invalid.
The spcimen should be retested.
